Invitrogen™ MELK Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5120104

Product Code. 17751504

Description

Positive test controls include: 293T, SKOV3, HT-29, HeLa, B cells, Mouse thymus, Mouse spleen, Rat testis. The target is usually found in the following locations: Cell membrane, Peripheral membrane protein. Immunogen sequence: LCRTPANKLK NKENVYTPKS AVKNEEYFMF PEPKTPVNKN QHKREILTTP NRYTTPSKAR NQCLKETPIK IPVNSTGTDK LMTGVISPER RCRSVELDLN QAHMEETPKR KGAKVFGSLE RGLDKVITVL TRSKRKGSAR DGPRRLKLHY NVTTTRLVNP DQLLNEIMSI LPKKHVDFVQ KGYTLKCQTQ SDFGKVTMQF ELEVCQLQKP DVVGIRRQRL KGDAWVYKRL VEDILSSCKV.

MELK is a serine/threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics. MELK has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

Specifications

• Antigen: MELK
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MELK
• Gene Alias: AI327312; hMELK; HPK38; Kiaa0175; Maternal embryonic leucine zipper kinase; Melk; mKIAA0175; MPK38; OTTHUMP00000046113; pEg3 kinase; Pk38; Protein kinase Eg3; protein kinase PK38; RP11-8N6.1; Tyrosine-protein kinase MELK
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17279, 362510, 9833
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.83 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q14680, Q61846
• Gene Symbols: MELK
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 412-651 of human MELK (NP_055606.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG