Invitrogen™ MegaScript™ T7 Transcription Kit Plus

Product Code.	No. of Reactions	unitSize
31662351	25 Reactions	-
17984681	50 Reactions	50 reactions
17934741	1000 Reactions	1000 reactions

Product Code. 31662351 Supplier Invitrogen™ Supplier No. A5762225

Description

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA, is suitable for full- or partially-modified nucleotide substitution to produce modified RNA, and enables the use of different cap analogs.

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be translated into protein. Easily scale from RUO to GMP with the incorporation of TheraPure reagents, eliminating reoptimization during scale up.

Features of the MegaScript T7 Transcription Kit Plus include:

• Optimized to achieve high mRNA yields (>5 mg/mL)
• Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
• Includes GFP control template
• Made up of high quality reagents (TheraPure); eliminates the need to qualify new reagents during scale-up
• Available in two sizes: 50 and 1000 reactions

The MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.

Specifications

• Cell Type: Primary Cells, Stem Cells, iPSC, Established Cell Lines, Hard-to-Transfect Cells
• Final Product Type: In vitro Transcribed RNA
• Format: Kit
• Isolation Technology: Silica Spin Column
• Purification Target: In vitro Transcribed RNA
• Sample Type: In vitro Transcribed RNA, Total RNA
• No. of Reactions: 25 Reactions
• Product Type: IVT mRNA kit
• Quantity: 25 Reactions

For Research Use Only.