missing translation for 'onlineSavingsMsg'
Få mere at vide
  1. Home
  2. Products
  3. Biochemical Reagents
  4. Transcription and Translation Reagents
  5. Invitrogen™ MegaScript™ T7 Transcription Kit Plus

Invitrogen™ MegaScript™ T7 Transcription Kit Plus

Artikelnummer. 17984681
Klik for at se tilgængelige muligheder
No. of Reactions:
1000 Reactions
25 Reactions
50 Reactions
Pakningsstørrelse:
1000 reactions
50 reactions
Denne vare kan ikke returneres. Se returpolicy

Artikelnummer. 17984681

Brand: Invitrogen™ A57622

Please to purchase this item. Need a web account? Register with us today!

467.20 GBP valid until 2025-12-31
BEST PRICE on Promo! Use promo code "24071" to get your promotional price.
Denne vare kan ikke returneres. Se returpolicy

Beskrivelse

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA, is suitable for full- or partially-modified nucleotide substitution to produce modified RNA, and enables the use of different cap analogs.

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be translated into protein. Easily scale from RUO to GMP with the incorporation of TheraPure reagents, eliminating reoptimization during scale up.

Features of the MegaScript T7 Transcription Kit Plus include:

  • Optimized to achieve high mRNA yields (>5 mg/mL)
  • Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
  • Includes GFP control template
  • Made up of high quality reagents (TheraPure); eliminates the need to qualify new reagents during scale-up
  • Available in two sizes: 50 and 1000 reactions

The MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.

TRUSTED_SUSTAINABILITY

Tekniske data

Cell Type HEK293, 293, HeLa, CHO-K1, CHO, Jurkat, Primary Human T Cells, Primary Cells, Stem Cells, iPSC, NIH-3T3, HepG2, A549, HUVEC
Final Product Type In vitro Transcribed RNA
Format Kit
Isolation Technology Silica Spin Column
Purification Target In vitro Transcribed RNA
Sample Type In vitro Transcribed RNA, Total RNA
No. of Reactions 50 Reactions
Quantity 50 Reactions

For Research Use Only.

Korrektion af produktindhold

Dit input er vigtigt for os. Udfyld denne formular for at give feedback relateret til indholdet på dette produkt.

Produkttitel

Ved at klikke på Send, anerkender du, at du kan blive kontaktet af Fisher Scientific med hensyn til den feedback, du har givet i denne formular. Vi deler ikke dine oplysninger til andre formål. Alle angivne kontaktoplysninger skal også vedligeholdes i overensstemmelse med vores Privatlivspolitik.

Mange tak! Din feedback er blevet sendt. Hos Fisher Scientific arbejder vi altid på at forbedre vores indhold for dig. Vi sætter pris på din feedback.