Gibco™ Medium 199, Hanks' Balanced Salts, powder

Medium 199, Hanks' Balanced Salts, powder

Product Code	Brand	Quantity
10165142	Gibco™ 10012011	1 L
11513387	Gibco™ 10012037	10 L

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO₂.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing
Gibco™ Medium 199 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Medium 199 contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Form: Powder
• With Additives: Glutamine, Hanks’ Balanced Salt solution (HBSS), HEPES, Phenol Red
• For Use With (Application): For cell culture, mammalian cell culture, vaccine production and In-vitro cultivation

• Content And Storage: 2 to 8°C
• Product Type: Medium 199 Hanks' Balanced Salts
• Without Additives: No Sodium Bicarbonate
• pH: 7 to 7.4