Gibco™ Medium 199, Earle's Salts (10X)

Product Code. 11500566

Brand: Gibco™ 21180021

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

With: Phenol Red, L-glutamine

Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Concentrated forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

• Cell Type: Mammalian Cells
• Classification: Animal Origin
• Concentration: 10 X
• Form: Liquid
• Product Type: Medium 199
• Sterility: Sterile-filtered
• With Additives: Glutamine, Phenol Red, Earle's Balanced Salt Solution (EBSS), Sodium Pyruvate
• Without Additives: No HEPES, No Sodium Bicarbonate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Osmolality: 2400 - 2900 mOsm/kg
• pH: 2 to 2.8
• Content And Storage: Storage conditions: 2-8° C. Protect from light.
  Shipping conditions: Ambient.
  Shelf life: 12 months from date of manufacture.
• Culture Environment: CO₂
• Culture Type: Mammalian Cell Culture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.