MCPT-1 (mMCP-1) Rat anti-Mouse, Clone: RF6.1, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-5503-82

100UG:ANTI-MOUSE MCPT-1 (MMCP-1) PURIFIED 100 UG

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15217427

Quantity Price
1 £ 138.0 / 100µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Antigen MCPT-1 (mMCP-1)
Applications Enzyme Linked Immunosorbent Assay
Applications Immunocytochemistry
Applications Immunohistochemistry
Clone RF6.1
Concentration 0.5mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Mast cell protease-1
Host Species Rat
Isotype IgG1
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The monoclonal antibody RF6 recognized MCPT-1 (Mast Cell Protease-1) which is a ß-chymase, a type of serine protease stored and secreted in a tissue-specific manner by mucosal mast cells. MCPT-1 is a chymotryptic protease, referring to its ability to cleave proteins and peptides after an aromatic amino acid. It shares 74% sequence homology with its rat counterpart, rat Mast Cell Protease-II (rMCP-II) and has no direct human counterpart.

MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCP-II, possibly by cleaving the proteins at tight junctions between cells.