Gibco™ MCDB 131 Medium, no glutamine

Code produit.	Quantity	unitSize
11513407	500 mL	500mL

Code produit. 11513407 missing translation for 'mfr' Gibco™ missing translation for 'supplierNo' 10372019

Description

MCDB 131 Medium, no glutamine contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins.

Using MCDB 131

MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Dual-site cGMP manufacturing

For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.

This MCDB 131 Medium is manufactured as follows:

With: Phenol Red, Sodium pyruvate

Without: L-glutamine, HEPES

The complete formulation is available.

Using MCDB 131

MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Dual-site cGMP manufacturing

For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Spécification

• Cell Type: Human Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells
• Classification: cGMP, Chemically-defined, Serum-free
• Concentration: 1 X
• Form: Liquid
• Product Type: MCDB 131 Medium
• Serum Level: Serum-free
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: Low Glucose, Phenol Red, Sodium Pyruvate
• Without Additives: No Glutamine, No HEPES, No Antibiotics
• Product Line: Gibco
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2–8°C (protect from light)
  Shelf life: 12 months from date of manufacture
  
  
• Culture Type: Mammalian Cell Culture

For Research Use Only. Not for use in diagnostic procedures.