Gibco™ McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement

Product Code.	Quantity	unitSize
11504476	10 x 500 mL	Pack of 10
11594466	500 mL	500mL

Product Code. 11504476

Brand: Gibco™ 36600088

Description

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

With: High Glucose, GlutaMAX™, Bacto-peptone, Phenol Red

Without: Sodium Pyruvate

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO₂ incubator.

Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Specifications

• Form: Liquid
• Product Type: McCoy's 5A (Modified) Medium
• With Additives: High Glucose, GlutaMAX, Bacto-peptone, Phenol Red
• Without Additives: No HEPES, No Sodium Pyruvate
• For Use With (Application): Mammalian cell culture
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 10 x 500 mL
• Certifications/Compliance: ISO 13485 standards, FDA
• pH: 6.9 to 7.1
• Description: McCoy's 5A (Modified) Medium, 10 x 500mL, Liquid, Phenol Red Indicator, With High Glucose, GlutaMAX™, Bacto-Peptone, Phenol Red, For Mammalian Cell Culture