Invitrogen™ MARVELD2 Recombinant Rabbit Monoclonal Antibody (54H19L38)

Rabbit Recombinant Monoclonal Antibody

Brand:  Invitrogen™ 700191

Product Code. 15386944

Description

This antibody is predicted to react with chimpanzee, mouse, rat, bovine and Xenopus based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Tight junctions form an important barrier of paracellular transport in epithelial cells. Sealing of two adjacent cells at bicellular tight junctions, a point where three adjacent cells are in contact with each other. Tricellulin is the first protein identified that specifically concentrates in tricellular tight junctions. This protein has four membrane spanning domains, similarly to claudins. Tricellulin expression is high in epithelium-derived tissues, such as small intestine, kidney and lung. Functional evidence for the role of tricellulin in tight junction formation comes from siRNA studies, where suppression of its expression leads to compromised epithelial barrier and tight junction formation. Loss of function in tricellulin mutants missing all or most of a conserved region in the cytosolic domain which binds to the cytosolic scaffolding protein ZO-1, indicate that interaction with other known tight junction proteins plays an important role for the function of tricellulin.

Specifications

• Antigen: MARVELD2
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: Q8N4S9
• Gene Symbols: MARVELD2
• Immunogen: A recombinant protein corresponding to amino acids 369-558 of Q8N4S9.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 54H19L38
• Conjugate: Unconjugated
• Gene: MARVELD2
• Gene Alias: BC003296; DFNB49; MARVD2; MARVEL (membrane-associating) domain containing 2; MARVEL domain containing 2; MARVEL domain-containing protein 2; Marveld2; Mrvldc2; Tric; Trica; Tricb; Tricc; Tricellulin
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 153562
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid