Marginal Zone B Cells, eFluor 660, clone: HIS57, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 50-9489-80

Product Code. 15570097

Description

Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.

Description: The monoclonal antibody HIS57 recognizes an unknown antigen expressed by rat marginal zone B cells. Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.Applications Reported: This HIS57 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This HIS57 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This HIS57 antibody has also been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Marginal Zone B Cells
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
• Clone: HIS57
• Conjugate: eFluor 660
• Gene Alias: MZB1, MZ-B
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.