Gibco™ MAPKAPK3 Recombinant Human Protein, Inactive

MAPKAPK3 Recombinant Human Protein, Inactive

Brand:  Gibco™ PV3300

Product Code. 11533417

Description

MAPKAPK3 functions as an integrative element of signaling in both mitogen and stress responses.

Specifications

• Accession Number: NP_004626
• For Use With (Application): Cell Viability, Pharma and Biopharma, Cell Proliferation and Function, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay
• Molecular Weight (g/mol): 47.3kDa
• Quantity: 100μg
• Regulatory Status: For research use only. Not for use in diagnostic procedures
• Gene Alias: MAPKAPK3
• Assays: Both the active and inactive enzymes were diluted in enzyme dilution buffer and the reaction was set up in two stages. In stage one, MAPKAPK3, inactive (3.33μg/mL final concentration) was incubated with MAPK14 (PV3304) (0.83μg/mL final concentration) or with dilution buffer for basal MAPKAPK3, inactive activity in 50mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 2mM DTT, 0.01% NP-40, 500μM ATP for 30 minutes at 30°C. In stage two, 5μL of the above reaction mix was transferred to 25μL of reaction mix containing 50mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 2mM DTT, 0.01% NP-40, 500μM ATP, 33μM MAPKAPK3 kinase substrate peptide and trace [32P]-γ-ATP and incubated for 10 minutes at 30°C
• Product Type: MAPKAPK3 Recombinant Human Protein
• Species: Human
• Expression System: Baculovirus
• Protein Subtype: Serine/threonine kinase

• Concentration: 0.98mg/mL
• Gene ID (Entrez): 7867
• Name: MAPKAPK3 Recombinant Human Protein
• Storage Requirements: –80°C
• Packaging: 1 Each
• Common Name: MAPKAPK3 Recombinant Human Protein
• KinaseFamily: MAPKAPK
• Specific Reactivity: ∽2% activity as compared to the active form of the kinase
• Protein Tag: His Tag
• Protein Family: Kinase and inhibitors
• Purity or Quality Grade: 95% by SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.