Invitrogen™ Human MAP3K3 (MEKK3), GST Tag Recombinant Protein

Product Code. 15498563

Brand: Invitrogen™ PR7352A

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length

MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks. Northern blot analysis revealed a 4. 6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Specifications

• Accession Number: Q99759
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris with 0.02% Triton X-100, 0.5 mM EDTA, 150 mM NaCl, 2 mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 4215
• Molecular Weight (g/mol): 99.8 kDa
• Name: Human MAP3K3 (MEKK3), GST Tag
• Quantity: 100 μg
• Regulatory Status: RUO - research use only
• Gene Alias: AW548911; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; Mekk3; mitogen activated protein kinase kinase kinase 3; mitogen-activated protein kinase kinase kinase 3; mKIAA4031
• Common Name: MEKK3
• KinaseFamily: STE11 Kinase Family
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Insect cells
• Form: Liquid