Invitrogen™ Human MAP2K1 (MEK1) [S218D, S222D], His Tag Recombinant Protein

Product Code. 11509296

Brand: Invitrogen™ P3099

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length

MAP2K1 (MEK1) kinase is a dual-specific protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. This mutant has been activated by substituting serines 218 and 222 with aspartic acid to mimic the phosphorylated state. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris with 0.05% NP-40, 0.05 mM EDTA, 150 mM NaCl, 1 mM DTT, 50% glycerol and no preservative; pH 7.5
• Molecular Weight (g/mol): 49.2 kDa
• Name: Human MAP2K1 (MEK1) [S218D, S222D], His Tag
• Quantity: 10 μg
• Regulatory Status: For research use only. Not for use in diagnostic procedures
• Common Name: MEK1 (S218D, S222D Mutant)
• KinaseFamily: STE7
• Product Type: MAP2K1 (MEK1) [S218D] [S222D] Recombinant Human Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Baculovirus
• Protein Family: Kinase and inhibitors
• Form: Liquid
• Protein Subtype: Serine/threonine kinase