Eurofins DiscoverX MAP Kinase 2/Erk2 Human Protein, Unactive

Product Code. 17340314

Brand: Eurofins DiscoverX 14536

Description

Unactive, N-terminal GST-tagged, recombinant human full length MAP Kinase 2, for use in Kinase Assays.

MAP Kinase 2/Erk2 Protein is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).

GenBank NM_002745. The cDNA sequence corresponds to a humanised version of the mouse MAP Kinase 2 coding sequence describ4ed inGenBank D10939. At the protein level the sequence is identical toGenBank NM_002745.

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 50% glycerol, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Liquid at -20°C.
• Molecular Weight (g/mol): 67.8 kDa
• Quantity: 50 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal GST
• Expression System: Recombinant enzyme expressed in E. coli cells
• Protein Form: Full-length
• Purity or Quality Grade: ≥78%
• Protein: MAP Kinase 2/Erk2, unactive