IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion

Product Code. 15653858

Brand: IBA Lifesciences 55033001

Beskrivning

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

pCSG-IBA mammalian expression vectors

• Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
  • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
  • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
• Stable cell lines can be selected by the neomycin resistance gene (NeoR).
• In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Specifikationer

• Constitutive or Inducible System: Constitutive
• Marker Gene: Ampicillin
• Promoter: CMV
• Product Type: Vector
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Protein Tag: His Tag (6x)
• Quantity: 5 μg
• Vector: pCSG-IBA33
• For Use With (Application): Episomal expression (oriP, EBNA-1)