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Invitrogen™ eBioscience™ MagniSort™ Human CD4 T cell 2-Step Enrichment Kit
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Quantity:
200 Tests
Unit Size:
200 tests
Description
Includes
- MagniSort Anti-Human CD14 Biotin : 200 tests, 20μL/test
- MagniSort Anti-Human CD4 Biotin : 200 tests, 20μL/test
- MagniSort Positive Selection Beads A
- Method
- Monocytes are bound by Anti-Human CD14 antibody and then magnetic beads
- When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step
- In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads
- Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted
- After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3
Specifications
Specifications
| Content And Storage | 2° to 8°C. Do not freeze. |
| For Use With (Application) | Magnetic Cell Separation |
| Format | Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer |
| Quantity | 200 Tests |
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