Applied Biosystems™ MagMAX™ mirVana™ Total RNA Isolation Kit

Product Code. 15422143

Brand: Applied Biosystems™ A27828

Description

The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types.

Scalable isolation of RNA (including small RNAs)

The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.

The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX mirVana Total RNA Isolation Kit include:

• Automation-ready, phenol-free extraction
• Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
• Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays

One kit to isolate true total RNA from a diverse set of sample types

Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering “specialized” kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples.

The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:

• Serum/plasma
• Whole blood
• Tissue
• Cell culture
• Urine

Benefits of MagMAX magnetic bead–based purification

Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Specifications

• Content And Storage:

  Box 1 (store at -15 to -25°C)
  • 1 x 0.48 mL Proteinase K, 20 mg/mL
  • 1 x 0.96 mL Lysis/Binding Enhancer
  • 1 x 0.2 mL TURBO DNase, 20 U/uL
  
  Box 2 (store at room temperature)
  • 1 x 115 mL Lysis Buffer
  • 1 x 4.4 mL PK Digestion Buffer
  • 1 x 2 mL RNA Binding Beads
  • 1 x 20 mL Wash Solution 1 Concentrate
  • 1 x 60 mL Wash Solution 2 Concentrate
  • 1 x 4.8 mL Rebinding Buffer
  • 1 x 4.8 mL MagMAX TURBO DNase Buffer
  • 1 x 9.6 mL Elution Buffer
  • 1 Processing Plate
  • 2 Elution Plates
  • 4 Plate Covers

• Isolation Technology: Magnetic Bead
• Purification Time: Up to 1.5 hr.
• Elution Volume: 20 to 100 μL
• Sample Type: Cells, Plasma, Serum, Tissue, Urine, Whole Blood
• Final Product Type: Total RNA
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Equipment): KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
• Quantity: 96 reactions
• Shipping Condition: Box 1: Dry Ice
  Box 2: Room Temperature
• Starting Material Amount: Cells: ≤10⁶
  Plasma: ≤100 μL
  Serum: ≤100 μL
  Tissue: ≤50 mg
  Urine: ≤250 μL
  Whole Blood: ≤50 μL
• Target: Total RNA, mRNA, siRNA, miRNA
• Yield: Cells: ≤32 μg RNA per 50,000 cells
  Tissue: ≤500 μg per 5 mg

Safety and Handling

Product Identifier

• MagMAX mirVana Total RNA Isolation Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H312-Harmful in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash face, hands and anyexposed skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Thiocyanic acid, Proteinase

For research use only. Not for use in diagnostic procedures.