MAdCAM1 Monoclonal Antibody (MECA-367), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5997-050

Product Code. 17111432

Description

The MECA-367 monoclonal antibody reacts with mouse mucosal vascular addressin-1 (MAdCAM-1), a 50-60 kDa member of the Ig superfamily. This antigen is expressed in mucosal lymphoid tissue and lamina propria and is a counter-receptor for CD62L and CD49d. MAdCAM-1 is involved in homing of subpopulation of memory T cells to the gut lamina propria, and recirculation of naive lymphocytes in Peyer's patches and mesenteric lymph nodes. Applications Reported: MECA-367 can be used in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, blocking in adhesion assays, immunoprecipitation and immunoblotting (WB). Applications Tested: The MECA-367 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in other applications. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

MAdCAM1 Monoclonal antibody specifically detects MAdCAM1 in Mouse samples. It is validated for Flow Cytometry, Functional Assay, Neutralization

Specifications

• Antigen: MAdCAM1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q61826
• Host Species: Rat
• Quantity: 50 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: MECA-367
• Conjugate: Functional Grade
• Gene: MADCAM1
• Gene Alias: AV211525; hMAdCAM-1; MACAM1; Madcam1; MAdCAM-1; mMAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1; rMAdCAM-1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17123
• Content And Storage: 4° C
• Form: Liquid