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  6. MAdCAM1 Monoclonal Antibody (MECA-367), Functional Grade, eBioscience™, Invitrogen™

MAdCAM1 Monoclonal Antibody (MECA-367), Functional Grade, eBioscience™, Invitrogen™

Product Code. 17111432
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Product Code. 17111432 Supplier Affymetrix eBioscience Supplier No. 165997050

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Description

Rat Monoclonal Antibody

The MECA-367 monoclonal antibody reacts with mouse mucosal vascular addressin-1 (MAdCAM-1), a 50-60 kDa member of the Ig superfamily. This antigen is expressed in mucosal lymphoid tissue and lamina propria and is a counter-receptor for CD62L and CD49d. MAdCAM-1 is involved in homing of subpopulation of memory T cells to the gut lamina propria, and recirculation of naive lymphocytes in Peyer's patches and mesenteric lymph nodes. Applications Reported: MECA-367 can be used in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, blocking in adhesion assays, immunoprecipitation and immunoblotting (WB). Applications Tested: The MECA-367 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in other applications. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

MAdCAM1 Monoclonal antibody specifically detects MAdCAM1 in Mouse samples. It is validated for Flow Cytometry, Functional Assay, Neutralization
TRUSTED_SUSTAINABILITY

Specifications

Antigen MAdCAM1
Applications Flow Cytometry, Functional Assay, Neutralization
Classification Monoclonal
Clone MECA-367
Concentration 1 mg/mL
Conjugate Functional Grade
Formulation PBS with no preservative; pH 7.2
Gene MADCAM1
Gene Accession No. Q61826
Gene Alias AV211525; hMAdCAM-1; MACAM1; Madcam1; MAdCAM-1; mMAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1; rMAdCAM-1
Host Species Rat
Purification Method Affinity chromatography
Quantity 50 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 17123
Target Species Mouse
Content And Storage 4° C
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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