Invitrogen™ M-CSF Monoclonal Antibody (2D10)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA515599

Product Code. 11375563

Description

MA5-15599 targets CSF1 in IHC and WB applications and shows reactivity with Human samples. The MA5-15599 immunogen is purified recombinant fragment of human CSF1 expressed in E. Coli. MA5-15599 detects CSF1 which has a predicted molecular weight of approximately 60kDa.

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

Specifications

• Antigen: M-CSF
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.03% sodium azide
• Gene Accession No.: P09603
• Gene Symbols: Csf1
• Immunogen: Purified recombinant fragment of human CSF1 expressed in E. Coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 1435
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 2D10
• Conjugate: Unconjugated
• Gene: Csf1
• Gene Alias: C87615; colony stimulating factor 1; colony stimulating factor 1 (macrophage); colony-stimulating factor-1 splice variant; CSF1; CSF-1; Csfm; H-MCSF; lanimostim; macrophage colony stimulating factor 1; macrophage colony stimulating factor alpha; macrophage colony stimulating factor beta; macrophage colony-stimulating factor; macrophage colony-stimulating factor 1; macrophage colony-stimulating factor beta; macrophage-colony stimulating factor alpha; MCSF; MCSF alpha; MCSFBETA; MGC31930; M-MCSF; op; osteopetrosis; Processed macrophage colony-stimulating factor 1
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1