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LYVE1, eFluor 660, clone: ALY7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0443-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15311870

  • £81.20 / 25µg


EU Stock 2
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Description

Description

Description: The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.Applications Reported: This ALY7 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections.Applications Tested: This ALY7 antibody has been tested by flow cytometric analysis of transfected cells or IHC on mouse tissue. This can be used at 1-10 μg/mL for IHC. It is recommended to titer the antibody in the application of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Lyve-1
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q8BHC0
Extracellular link domain containing 1, HAR, hyaluronic acid receptor, lymphatic vessel endothelial hyaluronan receptor 1, LYVE-1, XLKD1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Immunohistochemistry (Frozen)
ALY7
eFluor 660
Lyve1
Conjugated
Lyve1
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
114332
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