Invitrogen™ Lyo-ready SuperScript™ III Flash Reverse Transcriptase

Product Code. 31662844

Brand: Invitrogen™ A40006521

Description

Lyo-ready SuperScript III Flash RT is suitable for one-step RT-qPCR applications, helping ensure extremely short RT step time along with improved specificity, reliability, and thermostability.

Invitrogen Lyo-ready SuperScript III Flash Reverse Transcriptase joins the SuperScript product family as a reverse transcriptase designed for one-step RT-qPCR and point-of-care assay development. It is a novel SuperScript III Reverse Transcriptase variant, engineered for enhanced speed, thermostability, and inhibitor tolerance. Lyo-ready SuperScript III Flash Reverse Transcriptase provides enhanced sensitivity as low as five copies per reaction.

Benefits of Lyo-ready SuperScript III Flash Reverse Transcriptase include:

• Excellent choice for one-step RT-qPCR
• Fast cDNA synthesis (~1 minute) reduces the time to results
• Hot-start enables high-throughput applications, benchtop stability, and improved specificity of one-step RT-qPCR
• Thermostability up to 70°C allows elevated reaction temperatures and cDNA synthesis from complex RNA targets
• High inhibitor tolerance makes the system less dependent on RNA sample purity
• Commercial rights and customization enable product usage for commercial purposes

Hot start

Lyo-ready SuperScript III Flash Reverse Transcriptase is inactive at ambient temperatures due to the hot start mechanism, which enables room temperature reaction setup and improved specificity and consistency of RT-qPCR results. When used in conjunction with hot start DNA polymerase, such as Lyo-ready Platinum™ II Taq DNA Polymerase, a fully assembled master mix can be stored at room temperature for up to 24 hours, enabling high-throughput applications and compatibility with automation and point-of-care assay development. Due to enhanced specificity, this pair of enzymes also enables multiplex RT-qPCR of up to five targets.

Lyo-ready

Lyo-ready SuperScript III Flash Reverse Transcriptase is a glycerol-free enzyme that ensures compatibility with microfluidics, air-drying, and lyophilization technologies. For a full lyo-ready one-step RT-qPCR solution, we recommend using SuperScript III Flash Reverse Transcriptase together with Lyo-ready Platinum II Taq DNA polymerase. To learn more about Lyo-ready Enzymes for molecular diagnostics development visit the page thermofisher.com/lyo-ready.

OEM reverse transcriptase

To learn more about OEM and raw materials for molecular diagnostics, customization capabilities, and commercial rights, visit the page thermofisher.com/mdx.

Specifications

• Concentration: 60 U/μL
• Content And Storage: •Lyo-ready SuperScript™ III Flash Reverse Transcriptase, 2 kU
  •Store at -20°C.
• Format: Stand-alone Enzyme
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Fast
• Optimal Reaction Temperature: 60°C
• Quantity: 2 kU
• Ribonuclease H Activity: Reduced
• Shipping Condition: Dry Ice
• For Use With (Application): Virus Detection
• Hot Start: Build-In Hot Start
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Starting Material: RNA

For Research Use Only.