Ly-6G and Ly-6C Rat anti-Mouse, APC, Clone: RB6-8C5, BD

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15800759	BD Biosciences 561083	25 μg
15847018	BD Biosciences 553129	0.1 mg

Description

The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the “lineage cocktail” used in studies of hematopoietic lineages. The mAb 1A8 (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C.

Based on the comparison of the staining patterns of mAbs clones 1A8 and RB6-8C5 on total blood leukocytes, it is evident that mAb 1A8 stains the RB6-8C5-bright population, corresponding to Ly-6G-expressing granulocytes; whereas, the RB6-8C5-dim population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Please refer to the Technical Data Sheets for Cat. No. 551459 and 553128 for more detailed information.

Specifications

• Antigen: Ly-6G and Ly-6C
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Target Species: Mouse
• Immunogen: Not Reported
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: RB6-8C5
• Applications: Flow Cytometry
• Conjugate: APC
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Isotype: IgG2b κ
• Purification Method: Affinity Purified
• Description: Ly6c, Lymphocyte antigen 6C2; Lymphocyte antigen 6G, Ly6g, Gr-1

For Research Use Only.