Ly-49D Rat anti-Mouse, APC, Clone: eBio4E5 (4E5), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-5782-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15350750

Quantity Price
1 £ 164.47 / 100µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Applications Flow Cytometry
Conjugate APC
Regulatory Status RUO
Format Conjugated
Isotype IgG2a
Quantity 100μg
Clone eBio4E5 (4E5)
Species Reactivity Mouse
Host Species Rat
Primary or Secondary Primary
Antigen Ly-49D

The monoclonal antibody eBio4E5 (4E5) reacts with Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R. eBio4E5 is reported to have functional (activating) activity.