Ly-49C/I/F/H, PE, clone: 14B11, eBioscience™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5991-81

Product Code. 15259239

Description

Description: The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.Applications Reported: The 14B11 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Ly-49C/I/F/H
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q60653, Q60682, Q64329
• Gene Alias: Killer cell lectin-like receptor 3, 5E6, Lymphocyte antigen 49c, Nk2.1, T-cell surface glycoprotein Ly-49C, Klra3, Ly-49c, Ly49C, Killer cell lectin-like receptor 6, Lymphocyte antigen 49f, Ly-49f, T-cell surface glycoprotein Ly-49F, Klra6, Ly-49f, Ly49-f, Ly49F
• Host Species: Golden Syrian Hamster
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: 14B11
• Conjugate: PE
• Gene: Klra8
• Format: Conjugated
• Gene Symbols: Klra3, Klra6, Klra8
• Isotype: IgG
• Quantity: 50μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 16634, 16637, 16639

For Research Use Only.