Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, high ROX

Product Code.	Quantity	unitSize
13273159	250 x 20 μL Reactions	250 reactions
13283159	500 x 20 μL Reactions	500 reactions
13203169	1250 x 20 μL Reactions	1250 reactions
13213169	5000 x 20 μL Reactions	5000 reactions

Product Code. 13273159 Supplier Thermo Scientific™ Supplier No. K0331

Description

Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Thermo Scientific™ Luminaris Color Probe qPCR Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Thermo Scientific Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.

• Specificity no primer dimers

• Sensitivitity detects low copy number targets

• Wide linear range accurate quantification across 9 orders of magnitude

• Reproducibility and convenience ready-to-use 2X master mix

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Excellent qPCR performance
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Luminaris Color Probe High ROX qPCR Master Mix (2X) and Luminaris Color Probe Low ROX qPCR Master Mix (2X) contain different amounts of ROX passive reference dye for specific real-time platforms.

Includes:

• Luminaris Color Probe qPCR Master Mixes include Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye
• 40X Yellow Sample Buffer with a yellow dye
• Nuclease-free water

Recommended for:

Gene expression analysis; siRNA validation; Genotyping; Pathogen detection

Specifications

• Concentration: 2X (qPCR Master Mix), 40X (Buffer)
• Content And Storage: Contains:
  • 2 x 1.25 mL Luminaris Color Probe High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 μL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
  • 1 x 1.25 mL 40X Yellow Sample Buffer
  • 2 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• Description: Color Probe High ROX
• Detection Method: Primer-probe
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
• Product Line: Luminaris
• Product Type: qPCR Master Mix
• Quantity: 250 x 20 μL Reactions
• Sufficient For: 250 Reactions of 20 μL
• For Use With (Application): Gene Expression, Genotyping, siRNA Validation
• No. of Reactions: 250 Reactions
• Sample Type: DNA, dsDNA
• Volume: 2 x 1.25 mL

Frequently Asked Questions (FAQs)

Does dUTP in Luminaris qPCR master mix influence qPCR efficiency?

Luminaris qPCR master mixes have all dTTP substituted with dUTP. dUTP presence does not influence the reaction speed and efficiency of the master mix. 100% substitution of dTTP with dUTP facilitates an efficient clearance of the contamination from previous PCR reactions.

What enzyme is used in Luminaris qPCR Master Mixes?

Luminaris qPCR master mixes contain Hot Start Taq DNA Polymerase. This is a Taq DNA polymerase, which has been chemically modified by the addition of heat-labile groups to amino acid residues blocking enzyme activity. The Hot Start Taq DNA Polymerase has ultra low residual activity until thermally activated. Therefore reactions can be assembled at room temperature with no risk for the extension of non-specifically annealed primers or primer dimers and providing higher specificity for DNA amplification. The enzyme is activated by 10 minutes incubation at 95 degrees C.

Can I prepare a qPCR plate in advance and store it for later use when using Luminaris qPCR Master Mixes?

When using a hot start polymerase it is not critical to start reactions immediately after setup. The Hot Start Taq DNA Polymerase in Luminaris qPCR Master Mixes has ultra low residual activity until thermally activated. Therefore reactions can be assembled and stored at room temperature for up to 72 hours with no risk for the extension of non-specifically annealed primers or primer dimers.

For Research Use Only. Not for use in diagnostic procedures.