Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Código de producto.	Quantity	unitSize
15292465	0.75 mL	0.75mL
15282465	0.1 mL	0.1mL
15212475	1.5 mL	1.5mL
15222475	5 x 1.5 mL	7.5mL
15232475	15 mL	15mL

Código de producto. 15292465 Proveedor Invitrogen™ N.º de proveedor L3000008

Descripción

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Especificaciones

• Classification: Animal Origin-Free
• Content And Storage: Contains one vial of Lipofectamine™ 3000 Reagent and one vial of P3000TM Enhancer Reagent. Store at 2–8°C. Do not freeze.
• Cell Type: Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
• Format: 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
• Sample Type: Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
• Transfection Technique: Lipid-based Transfection
• For Use With (Application): Transfection
• Product Line: Lipofectamine
• Product Type: Transfection Reagent
• Quantity: 0.75 mL
• Serum Compatible: Yes

For Research Use Only. Not for use in diagnostic procedures.