Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit

Identify and characterize protein-DNA binding interactions by EMSA with this extraordinarily robust and sensitive system.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 20148X

1 SET LIGHTSHIFT(R) MOBILITY/GEL SHIFT ASSAY KIT(EMSA) 00 Store in Freezer at -20C

UNSPSC: 41105326

Code: PN

Additional Details:
Additional Details: Weight: 0.25000kg



Product Code. 10219663

Quantity Price
1 £ 136.0 / 1 set
EU Stock 28
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Description and Specification

Specification

For Use With (Application) Electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
Formulation

Multi-component kit with reagents for DNA-protein binding reactions

Includes 10X Binding Buffer, 1mL; Biotin-EBNA Control DNA, 50µL; Unlabeled EBNA DNA, 50µL; EBNA Extract, 125µL; Poly (dI·dC), 125µL; 50% Glycerol, 500µL; 1% NP-40, 500µL; 1 M KCl, 1mL; 100mM MgCl2, 500µL; 200mM EDTA pH 8.0, 500µL; 5X Loading Buffer, 1mL
No. of Reactions 100 binding reactions
Product Type LightShift EMSA Optimization and Control Kit
Quantity

100-rxn kit

The kit includes reagents for setting up and customizing protein:DNA binding reactions, a control set of DNA and protein extract to test the kit system, stabilized streptavidin-HRP conjugate to probe for the biotin-labeled DNA target, and an exceptionally sensitive chemiluminescent substrate module for detection.The principle for LightShift™ EMSA Detection is similar to a Western blot. Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel. The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV-crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate. The protocol from labeling to results can be accomplished in a single day. All you need to perform the assay are purified DNA target that has been end-labeled with biotin, the protein extract you wish to test, nylon membrane and basic electrophoresis equipment. DNA targets may be synthesized with 5' or 3' biotin labels or they may be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Part No. 89818). Nuclear, cytosolic or whole cell protein extracts may be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Part No. 78833).

Highlights:

  • Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity
  • Excellent for detecting low-abundance proteins in nuclear extracts
  • Sensitivity that surpasses radioactive and digoxigenin methods
  • Compatible with previously established binding conditions for popular DNA:protein interactions