LIF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen 500-P39BT-1MG

Product Code. 17825593

Description

AA Sequence of recombinant protein: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human LIF. Anti-Human LIF-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human LIF by sandwich ELISA (using 100 μL/well) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody,in conjunction with PeproTech Polyclonal Anti-Human LIF (500-P39) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LIF. Western Blot: To detect Human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human LIF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P39BT-1MG will be provided as 2 x 500 μg

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

Specifications

• Antigen: LIF
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Lif
• Gene Alias: CDF; cholinergic differentiation factor; cholinergic neuronal differentiation factor; d factor; DIA; differentiation inhibitory activity; differentiation-inducing factor; differentiation-stimulating factor; Emfilermin; hepatocyte-stimulating factor III; HILDA; human interleukin in DA cells; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; Melanoma-derived LPL inhibitor; MLPLI; myeloid leukaemia inhibitory factor
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3976
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P15018
• Gene Symbols: Lif
• Immunogen: E.coli-derived Recombinant Human LIF
• Quantity: 2 x 500μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized