Invitrogen™ Levonorgestrel (LNG) Competitive ELISA Kit

Competitive ELISA

Product Code	Brand	Sufficient For
15924224	Invitrogen™ EIALNGC	96 assays
17825217	Invitrogen™ EIALNGCX10	10 x 96 Assays

Description

The Levonorgestrel (LNG) ELISA quantitates LNG in serum,plasma,urine,dried fecal extracts,saliva,milk,or cell culture medium.
Principle of the method
The Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum,plasma,saliva,urine,dried fecal samples,milk,and tissue culture media samples. This kit measures total LNG in extracted serum,plasma,milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Levonorgestrel (4-Estren-17alpha-Ethynyl-18-homo-17beta-ol-3-one (LNG) is a synthetic steroid analogue.

Specifications

• Assay Sensitivity: 2.2 pg/mL
• Conjugate: HRP
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Intraassay CV: 9%
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Test Time: 1 hr. 20 min.

• Description: Levonorgestrel (LNG) Competitive ELISA Kit
• Detection Method: Fluorescence
• Sample Type: Fecal Extract, Milk, Plasma, Saliva, Serum, Supernatant, Urine, Water
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• Interassay CV: 9.1%
• Label or Dye: HRP
• Research Discipline: Reproductive Biology
• Target: Chemical
• Total Assay Time: 1 hr. 30 min.