Invitrogen™ LC3B Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA532254

Product Code. 13436777

Description

Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, mouse brain, Rat brain, HepG2 (untreated), HepG2 (3 μM Thapsigargin treatment for 12 hr), HepG2 (3 μM Thapsigargin treatment for 16hr), HepG2 (3 μM Thapsigargin treatment for 24hr), Huh7 (un-infected), Huh7 (HCV-infected), HeLa, HeLa (50 μM Chloroquine treatment for 24 hr). Predicted reactivity: Zebrafish (100%), Japanese Medaka (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.

Specifications

• Antigen: LC3B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MAP1LC3B2
• Gene Alias: 1010001C15Rik; Atg8; ATG8E; ATG8F; ATG8G; Autophagy-related protein LC3 B; autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3 beta; LC3A; LC3B; light chain 3; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; Map1alc3; MAP1BLC3; MAP1LC3; MAP1LC3A; MAP1LC3B; MAP1LC3B2; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; microtubule associated protein 1 light chain 3 beta 2; microtubule-associated protein 1 light chain 3; microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; Mpl3; RP11-346K17.1; wu:fb60g11; zbs559; zgc:56434
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100359928, 100462754, 643246, 64862, 67443, 81631
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.34 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300, pH 7
• Gene Accession No.: A6NCE7, Q62625, Q9CQV6, Q9GZQ8
• Gene Symbols: MAP1LC3B, MAP1LC3B2
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Insect, Mouse, Pig, Rat
• Product Type: Antibody
• Isotype: IgG