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Eurofins DiscoverX LATS1 Protein, Active
Description
LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
GenBank NM_004690.2 / GenBank BC038112.1
Specifications
Specifications
| Formulation | mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Molecular Weight (g/mol) | 90 kDa (LATS1) and 30 kDa (MOBKL1A) |
| Quantity | 10 μg |
| Species | Human |
| Recombinant | Recombinant |
| Protein Tag | N-terminal GST / N-terminal 6His |
| Expression System | Recombinant enzyme expressed in Sf21 insect cells |
| Protein Form | Truncated |
| Purity or Quality Grade | Combined ≥66% |
| Protein | LATS1, active |
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