LAP (Latency Associated peptide) Monoclonal Antibody (VB3A9), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 16-9823-80

Product Code. 15298827

Description

Description: This VB3A9 monoclonal antibody recognizes human latency-associated peptide (LAP), also known as the TGF-beta propeptide. Human LAP is a homodimer of 65-75 kDa that is non-covalently associated with TGF-beta and this complex is also referred to as the small latent complex (SLC). LAP can be covalently associated with latent TGF-beta binding proteins (LTBP) through disulfide bonds, forming the large latent complex (LLC). The association of LAP with TGF-beta facilitates its secretion and also renders TGF-beta inactive. VB3A9 has been reported to cross-react with non-human primate LAP, but does not cross-react with mouse LAP. Applications Reported: This VB3A9 antibody has been reported for use in immunoblotting (WB) and immunoprecipitation. Applications Tested: This VB3A9 antibody has been tested by immunoblot analysis of human platelets under reducing conditions at 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.

Specifications

• Antigen: LAP (Latency Associated peptide)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P01137
• Gene Symbols: TGFB1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7040
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot
• Clone: VB3A9
• Conjugate: Functional Grade
• Gene: TGFB1
• Gene Alias: CED, DPD1, LAP, TGFB, TGFbeta
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

For Research Use Only.