Thermo Scientific™ LanthaScreen™ TR-FRET TR beta Coactivator Assay Kit, goat

Product Code. 10668113

Brand: Thermo Scientific™ PV4686

Description

The LanthaScreen™ TR-FRET TR (thyroid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential TR beta ligands as agonists of ligand-dependent coactivator recruitment.

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15135:

The LanthaScreen™ TR-FRET TR (thyroid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential TR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR beta ligand-binding domain (TR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

To Assay:

When performing the LanthaScreen™ TR-FRET TR beta coactivator assay, TR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC₅₀ from a dose response curve of the compound. Based on the biology of the TR beta-coactivator peptide interaction, the ligand EC₅₀ is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:

The LanthaScreen™ TR-FRET TR beta Coactivator Assay Kit contains TR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

• Conjugate: Tb (Terbium)
• Description: LanthaScreen™ TR-FRET TR Beta Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Includes: TR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers
• Quantity: 800 x 20 μL assays
• Regulatory Status: For research use only (RUO)
• Packaging: 384-well Plate
• Content And Storage: Store in ultra-cold freezer (-68°C to -85°C).
• Assay Entry: Biochemical coregulator interaction
• Readout: End Point
• Shipping Condition: Dry Ice
• Target Entry: T3RB, TR beta, THRB, NR1A2
• Product Line: LanthaScreen™
• For Use With (Application): Co-Factor Interaction Assay, TR-FRET
• Ligand: TR Beta
• Product Type: TR-FRET TR Beta Coactivator Assay Kit
• No. of Assays: 800 x 20 μL Assays

For Research Use Only. Not for use in diagnostic procedures.