Thermo Scientific™ LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit, goat

Product Code. 10286503

Brand: Thermo Scientific™ PV4688

Description

The LanthaScreen™ TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment.

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15137:

The LanthaScreen™ TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RAR beta ligand-binding domain (RAR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

When running the LanthaScreen™ TR-FRET RAR beta coactivator assay in agonist mode to identify agonist compounds, RAR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC₅₀ from a dose response curve of the compound. Based on the biology of the RAR beta-coactivator peptide interaction, this ligand EC₅₀ is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Specifications

• Conjugate: Tb (Terbium), FITC (Fluorescein)
• Description: LanthaScreen™ TR-FRET RAR Beta Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, FITC (Fluorescein), Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Includes: RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers
• Quantity: 800 x 20 μL assays
• Regulatory Status: For research use only (RUO)
• Packaging: 384-well Plate
• Content And Storage: The LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit contains RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers.
  
  Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
• Assay Entry: Biochemical coregulator interaction
• Readout: End Point
• Shipping Condition: Dry Ice
• Target Entry: RAR beta, NR1B2, RARB
• Product Line: LanthaScreen™
• For Use With (Application): Co-Factor Interaction Assay, TR-FRET
• Ligand: PAR beta
• Product Type: TR-FRET RAR Beta Coactivator Assay Kit
• No. of Assays: 800 x 20 μL Assays

For Research Use Only. Not for use in diagnostic procedures.