Thermo Scientific™ LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR).

Brand:  Thermo Scientific™ A15142

Product Code. 15333806

Description

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4839:

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound's ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, 'addition-only' format.

Specifications

• Color: Green
• Description: LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, Rabbit, 800 x 20μL Assays, Green, 384-Well Plate Format, Fluorescent Detection Method, Nuclear Receptors Druggable Target, Tb (Terbium) Label, TR-FRET Readout, Nuclear Receptor Assay
• For Use With (Equipment): Microplate Reader
• Ligand Coupling Method: Competitive ligand binding
• Regulatory Status: For research use only. Not for use in diagnostic procedures
• Storage Buffer: TR-FRET PXR(SXR) assay buffer
• Assay Category: Biochemical Assay
• Readout: TR-FRET (Binding)
• For Use With (Application): Nuclear Receptor Assay, TR-FRET
• Product Type: TR-FRET PXR (SXR) Competitive Binding Assay Kit

• Conjugate: Tb (Terbium)
• Detection Method: Fluorescence
• Includes: Tb-anti-GST Antibody (Rabbit), PXR(SXR)-LBD, GST, fluormone PXR(SXR) green, TR-FRET PXR(SXR) assay buffer, 1M DTT
• Quantity: 800 x 20 μL Assays
• Packaging: 384-well Plate
• Content And Storage: • Tb-anti-GST Antibody (Rabbit) (store at -20°C)
  • PXR(SXR)-LBD, GST (-80°C)
  • Fluormone PXR(SXR) Green (-20°C)
  • TR-FRET PXR(SXR) Assay Buffer (4°C)
  • 1M DTT (-20°C or -80°C)
• Druggable Target: Nuclear Receptors
• Product Line: LanthaScreen™
• Ligand: PXR
• No. of Assays: 800 x 20 μL

For Research Use Only. Not for use in diagnostic procedures.