Thermo Scientific™ LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, rabbit

Product Code. 15313796

Brand: Thermo Scientific™ A15128

Description

The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment.

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655:

The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

In the LanthaScreen™ TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC₅₀ from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC₅₀ is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Specifications

• Color: Green
• Conjugate: Tb (Terbium)
• Description: LanthaScreen™ TR-FRET LXR Alpha Coactivator Assay Kit, 800 x 20μL Assays, Green, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification, Validation
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Includes: Tb-anti-GST antibody (rabbit), LXR alpha-LBD, GST, fluorescein coactivator peptide, TR-FRET coregulator buffer H, 1M DTT
• Quantity: 800 x 20 μL assays
• Regulatory Status: For research use only (RUO)
• Packaging: 384-well Plate
• Content And Storage: • Tb-anti-GST Antibody (Rabbit) (store at -20°C)
  • LXR alpha-LBD, GST (-80°C)
  • Fluoroscein Coactivator Peptide (-20°C)
  • TR-FRET Coregulatore Buffer H (4°C)
  • 1M DTT (-20°C or -80°C)
• Assay Category: Biochemical Assay
• Druggable Target: Nuclear Receptors
• Readout: TR-FRET (Activity)
• Product Line: LanthaScreen™
• For Use With (Application): Nuclear Receptor Assay, TR-FRET
• Ligand: LXR alpha
• Product Type: TR-FRET LXR Alpha Coactivator Assay Kit
• No. of Assays: 800 x 20 μL Assays

For Research Use Only. Not for use in diagnostic procedures.