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Thermo Scientific™ LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, rabbit

Product Code. 15313806
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Quantity:
800 x 20 μL assays
Unit Size:
1 set
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Product Code. 15313806

Brand: Thermo Scientific™ A15140

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This item is not returnable. View return policy

Description

The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment.

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4833:

The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.

To assay:

When running the LanthaScreen™ TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

TRUSTED_SUSTAINABILITY

Specifications

Color Green
Conjugate Tb (Terbium)
Description LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, 800 x 20μL Assays, Green, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Includes Tb-anti-GST antibody (rabbit), FXR-LBD, GST, fluorescein coactivator peptide, TR-FRET coregulator buffer G, 1M DTT
Quantity 800 x 20 μL assays
Regulatory Status For research use only (RUO)
Packaging 384-well Plate
Content And Storage • Tb-anti-GST Antibody (Rabbit) (store at -20°C)
• FXR-LBD, GST (-80°C)
• Fluoroscein Coactivator Peptide (-20°C)
• TR-FRET Coregulatore Buffer G (4°C)
• 1M DTT (-20°C or -80°C)
Assay Category Biochemical Assay
Druggable Target Nuclear Receptors
Readout TR-FRET (Activity)
Product Line LanthaScreen™
For Use With (Application) Nuclear Receptor Assay, TR-FRET
Ligand FXR
Product Type TR-FRET FXR Coactivator Assay Kit
No. of Assays 800 x 20 μL
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For Research Use Only. Not for use in diagnostic procedures.

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