Thermo Scientific™ LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit, goat

Product Code. 10585813

Brand: Thermo Scientific™ PV4408

Description

The LanthaScreen™ TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement.

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15121:

The LanthaScreen™ TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).

How it works

The LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human ERR gamma ligand-binding domain (ERR gamma-LBD) that is tagged with glutathione-S-transferase (GST). To assay, ERR gamma-LBD is added to test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti- GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit contains ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

• Conjugate: Tb (Terbium)
• Description: LanthaScreen™ TR-FRET ERR Gamma Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Includes: ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers
• Quantity: 800 x 20 μL assays
• Regulatory Status: For research use only (RUO)
• Packaging: 384-well Plate
• Content And Storage: Store in ultra-cold freezer (-68°C to -85°C).
• Assay Entry: Biochemical coregulator interaction
• Gene ID (Entrez): 2104
• Readout: End Point
• Shipping Condition: Dry Ice
• Target Entry: ERR3, ESRRG, NR3B3
• Product Line: LanthaScreen™
• For Use With (Application): Co-Factor Interaction Assay, TR-FRET
• Ligand: ERR gamma
• Product Type: TR-FRET ERR Gamma Coactivator Assay Kit
• No. of Assays: 800 x 20 μL Assays

For Research Use Only. Not for use in diagnostic procedures.