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Thermo Scientific™ LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, goat
Description
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15141:
The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:
When running the LanthaScreen™ TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand's EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).
Specifications
Specifications
| Conjugate | Tb (Terbium) |
| Description | LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Microplate Reader |
| Includes | CAR-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, terbium-labeled anti-GST antibody, and buffers |
| Quantity | 800 x 20 μL assays |
| Regulatory Status | For research use only (RUO) |
| Packaging | 384-well Plate |
| Content And Storage | The LanthaScreen™ TR-FRET CAR Coactivator Assay Kit contains CAR-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C). |
| Assay Entry | Biochemical coregulator interaction |
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For Research Use Only. Not for use in diagnostic procedures.
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