Thermo Scientific™ LanthaScreen™ Tb-pMAP2K1 (pSer217/221) Antibody Kit

LanthaScreen™ Tb-pMAP2K1 (pSer217/221) Antibody Kit

Brand:  Thermo Scientific™ PV4813

Product Code. 10206313

Description

A member of the MAP Kinase family, MAP2K1 (mitogen-activated protein kinase kinase 1) is phosphorylated at Ser217/221 by a range of MAPK family members including Raf and B-Raf. For use in combination with the Fluorescein-MAP2K1 physiological protein substrate (PV4812) to evaluate inhibitors in medium to high throughput screening applications.

Specifications

• Applications: Kinase Assay, Enzyme and Protein Activity Assays, Kinase Assay
• Gene Alias: COT,EST,ESTF,FLJ10486,MEKK8,TPL2,Tpl-2,c-CO, CRAF,NS5,Raf-1,c-Ra, MEKK9,MLK1,PRKE, COT,EST,ESTF,FLJ10486,MEKK8,TPL2,Tpl-2,c-CO, MEKK7,TAK1,TGF1, MEKK9,MLK1,PRKE, ASK1,MAPKKK5,MEKK, MEKK10,MLK2,MS, MEKK11,MGC17114,MLK-3,MLK3,PTK1,SPR, CRAF,NS5,Raf-1,c-Ra,
• Quantity: 25 μg
• Research Discipline: Target and Lead Identification and Validation
• Gene ID (Entrez): 10454, 10746, 1326, 4215, 4217, 4293, 4294, 4296, 5894, 673, 6885
• Includes: 0.02% Sodium azide
• Antibody Molecular Weight: 150000 Da
• Format: 384-well Plate
• Reactivity: Human
• Readout: End Point
• Target Entry: MAP3K2, RAF1 (cRAF) Y340D Y341D, MAP3K10, MAP3K9, MAP3K7/MAP3K7IP1, MAP3K5, MAP3K11, BRAF V599E, BRAF, MAP3K8, MAP3K3
• Technique: TR-FRET

• Conjugate: Tb (Terbium)/FITC/Lanthanide Chelates
• Molecular Weight of Antigen: 150kDa
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Packaging: 1 Kit(s)
• Content And Storage: Terbium-labeled anti-MAP2K1 [pSer217/221] antibody in HEPES buffered saline. Store at -20°C.
• Assay Entry: Biochemical terbium phospho-specific activity
• Excitation/Emission: 340⁄490, 520
• Shipping Condition: Dry Ice
• Reactivity abrv: Hu
• Target Enzyme: Serine⁄Threonine Kinases
• Validated Kinases: BRAF, MAP3K2, MAP3K3, MAP3K5, MAP3K7, MAP3K8, MAP3K9, MAP3K10, MAP3K11

For Research Use Only. Not for use in diagnostic procedures.