Invitrogen™ LanthaScreen™ Tb-p4E-BP1 (pThr46) Antibody Kit

A part of the PI3K/AKT Pathway, 4E-BP1 (EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1) is a cytosolic protein that is phosphorylated at Thr46 by mTORC1 upon stimulation of the pathway.

Brand:  Invitrogen™ PV4757

Product Code. 10053143

Description

A part of the PI3K/AKT Pathway, 4E-BP1 (EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1) is a cytosolic protein that is phosphorylated at Thr46 by mTORC1 upon stimulation of the pathway.

For use in combination with the GFP-4E-BP1 physiological protein substrate (Cat. No. PV4759) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.

Specifications

• Antigen: Serine/threonine kinase
• Concentration: 0.52mg/mL
• Molecular Weight of Antigen: 150kDa
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Terbium-labeled anti-4E-BP1 [pThr46] antibody in HEPES buffered saline.
  
  Store at -20°C.
• Assay Entry: Biochemical terbium phospho-specific activity
• Excitation/Emission: 340/490, 520 nm
• Shipping Condition: Dry Ice
• Reactivity abrv: Hu
• Target Enzyme: Serine/Threonine Kinases
• Validated Kinases: FRAP1 (mTOR), FRAP1 (mTOR)

• Applications: Kinase Assay, Enzyme and Protein Activity Assays, Kinase Assay
• Conjugate: Tb (Terbium)/GFP (EmGFP)
• Quantity: 25 μg
• Research Discipline: Target and Lead Identification and Validation
• Packaging: 1 Kit(s)
• Antibody Molecular Weight: 150,000 Da
• Format: 384-well Plate
• Reactivity: Human
• Readout: End Point
• Target Entry: mTOR, FRAP1
• Technique: TR-FRET
• Product Line: LanthaScreen™

For Research Use Only. Not for use in diagnostic procedures.