Invitrogen™ LanthaScreen™ Tb-anti-GFP Antibody

LanthaScreen™ Tb anti-GFP Antibody is a terbium-labeled antibody recognizing green fluorescent protein (GFP).

Brand:  Invitrogen™ A13391

Product Code. 11330732

Description

LanthaScreen™ Tb anti-GFP Antibody is a terbium-labeled antibody recognizing green fluorescent protein (GFP). It can be used to determine GFP expression levels in any GFP expressing cell line with greater sensitivity than detecting the fluorescence intensity of the GFP protein alone.

With the LanthaScreen™ Tb anti-GFP Antibody You Can:

• Amplify GFP signal expression for more sensitive results
• Track GFP-target protein expression levels
• Easily build TR-FRET assays from any GFP expressing cell line

Detect Low Level GFP Expression

GFP is a commonly used reporter for many types of research. In some instances, the level of GFP-target protein expression is not high enough to generate a suitably strong fluorescence intensity signal. The LanthaScreen™ Tb anti-GFP Antibody can help overcome this problem through the use of TR-FRET assays. Since TR-FRET is a ratiometric readout and does not rely on the intensity of the signal alone, low levels of GFP expression are not a problem.

Use TR-FRET to Track Protein Expression Levels

Using the LanthaScreen™ Tb anti-GFP Antibody in conjunction with a cell line expressing GFP-target fusion protein can allow development of TR-FRET assays for protein accumulation or degradation. Following titration of a compound that induces or inhibits the expression of your GFP-target protein, LanthaScreen™ Tb anti-GFP can be added to the resultant cell lysates and the TR-FRET ratios compared to determine the relative effect of the compound on the GFP-target protein expression level.

Create New TR-FRET Assays from Any GFP Expressing Cell Line

Because the terbium label on the LanthaScreen™ Tb anti-GFP Antibody can be used as a donor for TR-FRET with GFP, any GFP-expressing cell line can be used with the antibody to build a TR-FRET assay. The antibody will bind to GFP in a cell lysate sample, and a simple ratiometric measurement can determine the differences in GFP expression levels between different samples.

Specifications

• Antigen: GFP
• Concentration: 0.54mg/mL
• Quantity: 25 μg
• Research Discipline: Pharma and Biopharma
• Packaging: 1 Each
• Form: Liquid
• Target Molecule: GFP
• Product Line: LanthaScreen

• Applications: Kinase Assay
• Conjugate: Tb (Terbium)/Lanthanide Chelates
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Terbium-labeled anti-GFP antibody in HEPES buffered saline.
  
  Store at -20°C.
• Shipping Condition: Dry Ice
• Technique: TR-FRET

For Research Use Only. Not for use in diagnostic procedures.