Invitrogen™ Lamin B1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA579606

Product Code. 15945175

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human Daudi whole cell, human THP-1 whole cell, human HL-60 whole cell, rat lung tissue, mouse lung tissue. IHC: Human Mammary Cancer tissue IHC-F: Rat Intestine tissue. ICC/IF: SiHa cell, SiHa cell, A549 cell.

Lamin B1 is a key member of the nuclear lamina structure, encoded by the LMNB1 gene. It is part of the B-type lamins, ubiquitously expressed across all mammalian cells, providing structural integrity and mechanical support by forming a meshwork along the inner nuclear membrane. Lamin B1 is involved in maintaining the shape and stability of the nucleus, playing a critical role in cellular processes such as genome organization, DNA replication, and transcription regulation. The lamin filaments are type V intermediate filament proteins, enabling the nucleus to withstand mechanical stress and preserve its functionality. Lamin B1's expression and dynamics vary during cell differentiation, influencing gene regulation. Its location within the euchromatin-enriched section of the genome suggests potential roles in chromatin organization and the epithelial-to-mesenchymal transition. Governed by complex interactions with other proteins, lamin B1 is integral to cellular architecture and health, with variations in its levels linked to developmental and disease processes. Recent studies have highlighted the importance of understanding the role and dynamics of lamin B1 in human-induced pluripotent stem cells and germ layer cells, offering insights into differentiation-dependent changes and their implications for nuclear structure.

Specifications

• Antigen: Lamin B1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Lmnb1
• Gene Alias: 0710005A05Rik; ADLD; C-flanking peptide of NPY; CPON; fc06g01; I79_003938; lamin B1; lamin B1 L homeolog; lamin B1 protein; lamin-b; lamin-B1; Lamin-L(I); LMN; LMN2; LMNB; LMNB1; lmnb1 protein; lmnb1.L; MGC111419; Neuropeptide tyrosine; Neuropeptide Y; NPY; NPY02; prepro-neuropeptide Y; pro-neuropeptide Y; PYY4; RATNPY; RATNPY02; unnamed protein product; wu:fc06g01; XELAEV_18007951mg
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 116685, 16906, 4001
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P14733, P20700, P70615
• Gene Symbols: Lmnb1
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1 (570-586aa FHQQGTPRASNRSCAIM).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG