Invitrogen™ Lamin A/C Monoclonal Antibody (4A7), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15557606	Invitrogen™ 14-9688-80	25 μg
17130752	Invitrogen™ 14-9688-37	2 mg

Description

The monoclonal antibody 4A7 recognizes human, mouse, rabbit and hamster lamin A/C. Lamins are nuclear intermediate filament proteins that provide framework for the nuclear envelope, maintain cell morphology, and protect the nucleus from mechanical, thermal, and oxidative stresses. Lamins also play a role in nuclear assembly, chromatin organization, DNA replication, RNA transcription, cell signaling, and apoptosis. Lamin C is a splice variant of Lamin A and both are A-type lamins. Lamin A and C are crucial for skeletal and cardiac development and function. Defects in A-type lamins result in cardiomyopathy, muscular dystrophy, peripheral neuropathy, lipodystrophy, restrictive dermopathy, and progeroid disorders. This 4A7 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Specifications

• Antigen: Lamin A/C
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% Sodium Azide; pH 7.2
• Gene Alias: 70 kDa lamin; cb948; CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; I79_009616; IDC; lamin; lamin A; lamin A/C; lamin A/C L homeolog; lamin A/C-like 1; Lamin A+C mutant; Lamin AC; lamin a-c; lamin C; lamin C2; lamin-A; Lamin-A/C; LDP1; LFP; LGMD1B; LMN1; lmna; LMNA protein; lmna.L; lmna-A; LMNC; LMNL1; mutant 453W; Mutant lamin A/C; nuclear intermediate filament protein; nuclear lamin A; prelamin-A/C; PRO1; progerin mutant; Renal carcinoma antigen NY-REN-32; RP11-54H19.1; unnamed protein product; wu:fk66d12; XELAEV_18040808mg
• Gene Symbols: LMNA
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: LMNA

• Clone: 4A7
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rabbit, Hamster
• Gene Accession No.: P02545, P48678
• Gene ID (Entrez): 100343574, 100757316, 16905, 4000
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody