Thermo Scientific™ Klenow Fragment, exo-

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EP0422

MODIFYING ENZYME, DNA POLYMERASES, KLENOW FRAGMENT, exo-, 5u/µL (supplied with 5 x1mL of 10X reactio

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.02000kg



Product Code. 10399689

Quantity Price
1 £ 212.0 / 1500 units
EU Stock 11
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Description and Specification

Specification

Concentration 5U/µL
Inhibitors metal chelators, PPi, Pi (at high concentrations) (7)
Source E.coli cells with a cloned DNA fragment of the mutated polA gene
Molecular Weight 68kDa monomer
Units 1,500U

Highlights

  • Lacks 3'→5' exonuclease activity
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
  • Active in restriction enzyme, PCR, and RT buffers

Applications

  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • Strand displacement amplification (SDA)
  • DNA sequencing by the Sanger method

Note

Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion