Thermo Scientific™ Klenow Fragment

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Brand: Thermo Scientific™

Manufacturer Part Number: EP0054

MODIFYING ENZYME, KLENOW FRAGMENT, 2U/UL, LC,(suppliedwith 1mL of 10X reaction buffer) 300

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg

Product Code. 10450440

Quantity Price
1 £50.25 / 300 units
EU Stock 11
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Description and Specification


Concentration 2U/┬ÁL
Inhibitors metal chelators, PPi, Pi (at high concentrations) (8)
Source E.coli cells with a cloned fragment of the polA gene
Molecular Weight 68kDa monomer
Units 300 U


  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers


  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA