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Thermo Scientific™ Klenow Fragment (10 U/µL)

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Brand:  Thermo Scientific™ EP0051

39.87 GBP valid until 2021-12-22
Use promo code "16346" to get your promotional price.



Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10130800

  • £59.50 / 300 units

Estimated Shipment: 22-10-2021

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Description

Description

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA
Specifications

Specifications

300 U
Klenow Fragment
10 U/μL
DNA Polymerase I
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