Invitrogen™ Ki-67 Recombinant Rat Monoclonal Antibody (SolA15), Alexa Fluor™ Plus 555, Invitrogen™

Rat Recombinant Monoclonal Antibody

Product Code	Brand	Quantity
30019294	Invitrogen™ 740008TP555	50 μL
30019352	Invitrogen™ 740008TP55520UG	20 μL

Description

Ki-67 Recombinant Monoclonal antibody specifically detects Ki-67 in Human, Mouse samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin)

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This SolA15 antibody has been tested by immunohistochemistry of mouse spleen tissue and immunocytochemistry and flow cytometric analysis of HeLa cells. This may be used at 0.02-0.25 μg per test for flow cytometry. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser Filtration: 0.2 μm post-manufacturing filtered.

Specifications

• Antigen: Ki-67
• Concentration: 1.0 mg/mL
• Classification: Recombinant Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene Alias: antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: SolA15
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Conjugate: Alexa Fluor Plus 555
• Host Species: Rat
• Target Species: Human, Mouse
• Gene Accession No.: E9PVX6, P46013
• Gene ID (Entrez): 17345, 4288
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Mki67