Invitrogen™ Human Kallikrein 6 ELISA Kit

Product Code. 15475053

Brand: Invitrogen™ EHKLK6

Description

ELISA

The Human Kallikrein 6 (Hu KLK6) ELISA quantitates Hu KLK6 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu KLK6. Principle of the method The Human KLK6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Kallikreins, including Kallikrein 6, are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The KLK6 enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease.

Specifications

• Accession Number: Q92876
• Assay Range: 0.614 to 150 ng/mL
• Assay Sensitivity: 0.6 ng/mL
• Calibration Range: 0.6 ng/mL to 150 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: GCPR
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human KLK6. Other species not determined.
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5653
• Gene Symbol: Bssp,Klk7,PRSS18,PRSS9,SP59,hK6
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Neurobiology
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Bssp, Klk7, PRSS18, PRSS9, SP59, hK6
• Target: Kallikrein 6
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins