Eurofins DiscoverX JAK1 Protein, Active

Product Code. 17360784

Brand: Eurofins DiscoverX 14918

Description

Active, N-terminal GST-tagged, recombinant, human JAK1, amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Activated in vitro by auto-phosphorylation, for use in Kinase Assays.

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

GenBank NM_002227

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 5 mM β-glycerophosphate, 0.5 mM sodium orthovanadate, 50% v/v glycerol, 0.02% Triton™ X-100, 0.5 mM EDTA, 2 mM DTT. Frozen solution.
• Molecular Weight (g/mol): 61 kDa
• Quantity: 10 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal GST
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥57%
• Protein: JAK1, active